The next window will ask you to specify whether the T-test is an unpaired (independent) or paired (dependent) test. Select the ‘ t tests (and nonparametric tests)’ analysis and make sure the two datasets are ticked on the right window.

Here you need to tell GraphPad which test to perform. To perform a paired t-test, first go to ‘ Insert > New Analysis …’. “There is a difference in the number of viable cells before and after a 6-hour treatments with drug X”. “There is no difference in the number of viable cells before and after a 6-hour treatments with drug X”.

The data is entered into two columns labelled ‘before’ and ‘after’. Then select ‘ Enter paired or repeated measures data – each subject on a separate row’ as the ‘ Enter/import data’ choice.Īlternatively, you can go to ‘ File > New > New Data Table & Graph …’.įor this tutorial, I will use an example of comparing the number of viable cells before and after a 6-hour treatment with drug X.

Upon opening GraphPad Prism, select the ‘ Column’ type for the ‘ New Table & Graph’ option. To perform a paired (dependent) T-test in GraphPad Prism you will need to enter two groups of data into separate columns.

Graphpad prism 8 drug test